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Automated Sequential‐injection Chemiluminescence Determination of Glucosamine Sulphate via Luminol‐Hydrogen Peroxide System
Author(s) -
Alarfaj Nawal A.,
ElTohamy Maha F.
Publication year - 2013
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201300187
Subject(s) - chemistry , chemiluminescence , luminol , detection limit , hydrogen peroxide , reagent , chromatography , flow injection analysis , sodium hydroxide , relative standard deviation , nuclear chemistry , analytical chemistry (journal) , organic chemistry
A highly sensitive automated sequential‐injection chemiluminescence (SIA‐CL) method for determination of glucosamine sulphate (GLS) was developed. The goal of the present work is the evaluation of the enhancement effect of the investigated drug glucosamine sulphate on the chemiluminescence reaction between luminol and H 2 O 2 in alkaline medium of 1.0 × 10 −2 mol L −1 sodium hydroxide at pH 11. The experimental conditions affecting the CL reaction such as the sequence of the reagents, concentrations, flow rate and aspirated volumes of reactants were systematically investigated and optimized. Under optimum conditions 50 μL of 1.0 × 10 −3 mol L −1 luminol, 30 μL of a GLS test solution and 50 μL of 1.0 × 10 −2 mol L −1 H 2 O 2 were used and the luminescing zone was pushed into the detector at a flow rate 100 μL s −1 . The proposed method recorded high sensitivity, accuracy and simplicity that could be clarified as linear concentration range 1.0‐2000 n g mL −1 with rectilinear part (r = 0.9992, n = 9) and limit of detection 0.3 n g mL −1 , along with relative standard deviation 1.3%. It was found that the developed method can be used directly to determine the investigated drug GLS in its pharmaceutical dosage forms and in spiked serum and urine by diluting the samples for a 1000 fold. The obtained results were statistically analyzed and compared with those obtained by the reported method.