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Myoglobin Detection: Utilizing Functional Magnetic Hybrid and Silica Micro Particles and Simple Molding Based Micro Channel Fabrication
Author(s) -
Viswanathan Kaliyaperumal,
Haung ShiTin,
Chang ChiaYu,
Chan ChingJu,
Lee Y. C.
Publication year - 2013
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201200655
Subject(s) - myoglobin , chemistry , separator (oil production) , calibration curve , conjugated system , analytical chemistry (journal) , chromatography , detection limit , polymer , organic chemistry , physics , thermodynamics
A magnetic separator attached in a zig zag flow channel assembly has been fabricated to detect myoglobin on the micro particles. An anti‐myoglobin conjugated magnetic hybrid and myoglobin conjugated silica micro particles were prepared and used for myoglobin detection on channel. For the detections, the competitive assay principle was followed based on affinity interaction of anti‐myoglobin‐myoglobin. The caliberation curve was plotted based on the deposit percentage of myoglobin conjugated silica micro particles. The calibration curve of the myoglobin showed the linear range between 1 × 10 −8 ‐ 5 × 10 −11 (M) (R 2 = 0.9944). The minimum detectable concentration was 300 pg/mL. Hence all that concludes that, the application of zig zag flow channel with magnetic separator has offered new, simple and rapid approach for detecting myoglobin in whole blood samples with in 30 min.