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Nano‐Gold Modified Genosensor for Direct Detection of DNA Hybridization
Author(s) -
MousaviSani Seyedeh Zeinab,
Raoof Jahan Bakhsh,
Ojani Reza,
HamidiAsl Ezat
Publication year - 2013
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201200642
Subject(s) - chemistry , differential pulse voltammetry , detection limit , guanine , oligonucleotide , biosensor , dna–dna hybridization , hybridization probe , dna , oligomer restriction , carbon paste electrode , electrode , selectivity , colloidal gold , analytical chemistry (journal) , combinatorial chemistry , electrochemistry , cyclic voltammetry , nanotechnology , chromatography , gene , biochemistry , nanoparticle , nucleotide , materials science , catalysis
Abstract In this paper, nano‐gold modified carbon paste electrode (NGMCPE) was employed to develop an electrochemical DNA hybridization biosensor. The proposed sensor was made up by immobilization of 15‐mer single stranded oligonucleotide probe for detection of target DNA. Hybridization detection relies on the alternation in guanine oxidation signal following hybridization of the probe with complementary genomic DNA. The guanine oxidation was monitored using differential pulse voltammetry (DPV). Different factors such as activation potential, activation time and probe immobilization conditions were optimized. The selectivity of the sensor was investigated by non‐complementary oligonucleotides. Diagnostic performance of the biosensor was described and the detection limit was found 1.9 × 10 −13 M at the NGMCPE surface. All of the investigations were performed in both CPE and NGMCPE and finally their results were compared.