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Electrochemical Behaviors of Glucose Oxidase Based on Biocatalytic Deposition of Gold Nanoparticles
Author(s) -
He Yaping,
Zheng Jianbin
Publication year - 2013
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201200470
Subject(s) - glucose oxidase , chemistry , pyrolytic carbon , biosensor , colloidal gold , electrochemistry , electrocatalyst , electrode , electron transfer , linear range , detection limit , nanoparticle , nanotechnology , nuclear chemistry , chemical engineering , organic chemistry , chromatography , biochemistry , pyrolysis , materials science , engineering
Abstract Coupling nanotechnology with biocatalysis, a highly sensitive glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using monkshoodvine root–bark like carbon (MLC) as the platform for the biocatalytic deposition of AuNPs. The biosensor showed a linear range from 0.25 to 130 μM with a detection limit of 0.1 μM (S/N = 3) towards glucose and sensitivity of 3010 μA/mM. K app Mvalue was calculated to be 67.4 μM. Furthermore, the proposed AuNPs/GOx–MLC modified pyrolytic graphite electrode (AuNPs/GOx–MLC/PGE) achieved direct electron transfer of GOx. Γ* was calculated to be 2.79 × 10 −11 mol/cm 2 and k s was 1.79 s −1 . It also showed a remarkable electrocatalysis towards glucose.