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Chemiluminescence Determination of Gossypol by Oxidation with N‐Bromosuccinimide
Author(s) -
Han Suqin,
Hao Fang,
Wei Bei
Publication year - 2013
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201200464
Subject(s) - chemistry , gossypol , chemiluminescence , n bromosuccinimide , detection limit , dichlorofluorescein , cottonseed oil , chromatography , cottonseed , nuclear chemistry , organic chemistry , halogenation , biochemistry , food science , antioxidant
A chemiluminescence (CL) phenomenon was observed when gossypol was injected into a reaction mixture of N‐bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF). Based on this phenomenon, a rapid and sensitive method for the determination of gossypol was established. Under the optimum conditions, the linear range was from 1.0 × 10 −9 to 1.0 × 10 −6 M. The detection limit was 1.0 × 10 −10 M. The method has been applied to the determination of gossypol in cottonseed, cottonseed oil, pharmaceutical and biological fluids with satisfactory results. The possible CL reaction mechanism was discussed briefly.