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Sensitive Determination of 2‐Methoxyestradiol in Pharmaceutical Preparations and Biological Fluids by KMnO 4 ‐Na 2 SO 3 Chemiluminescence System
Author(s) -
Yao Hanchun,
Zhou Jie,
Zhang Min,
Xu Erjun,
Chen Juan,
Zhang Zhenzhong
Publication year - 2013
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201200352
Subject(s) - chemistry , chemiluminescence , detection limit , relative standard deviation , biological fluids , analytical chemistry (journal) , chromatography
A simple and novel flow‐injection chemiluminescence (FI‐CL) method was established for the determination of 2‐Methoxyestradiol (2‐ME) in pharmaceutical preparations and biological fluids. The method was based on the significant enhancement of the CL from the KMnO 4 ‐Na 2 SO 3 reaction by 2‐ME in acidic medium. Under optimized conditions, the CL intensity was correlated linearly with concentration of 2‐ME in the range of 5.0 × 10 −8 ‐5.0 × 10 −6 M (r = 0.9995). The detection limit (3σ) of 2‐ME was 7.5 × 10 −9 M and the relative standard deviation was 0.8% at 5.0 × 10 −7 M 2‐ME (n = 8). The proposed method was successfully applied for the flow‐injection CL determination of 2‐ME in pharmaceutical preparations and biological fluids with the recoveries from 92.4 to 106.8%. The possible CL reaction mechanism was also discussed briefly.