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The Isolation and Determination of Sulforaphane from Broccoli Tissues by Reverse Phase‐High Performance Liquid Chromatography
Author(s) -
Azizi Naser Seyed,
AmiriBesheli Behnam,
SharifiMehr Salman
Publication year - 2011
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201190143
Subject(s) - sulforaphane , glucoraphanin , myrosinase , glucosinolate , chemistry , brassica , isothiocyanate , chromatography , seedling , high performance liquid chromatography , food science , biochemistry , botany , biology
Sulforaphane is a cognate isothiocyanate of glucoraphanin, released by degradation of glucosinolate through myrosinase enzyme, when Brassica tissues are crushed or chewed. C 8 analytical column and mixture of water/acetonitrile 65/35 (ν/ν) as mobile phase were used to separate sulforaphane in broccoli tissues (e.g., 7‐day‐seedling, seed, floret and leave). Confirmation of sulforaphane in crude extract of broccoli tissues was monitored using gas chromatography‐mass spectroscopy (GC‐MS). The highest amount of sulforaphane (1216 μg/g dry weight) found in 7‐day‐seedling. This method is suitable for routine screening of plant materials and proposes a low cost and robust technique for the analysis of sulforaphane.