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A Novel Hydrogen Peroxide Biosensor Based on the PDDA‐GNPs/MWNTs Nanocomposite Matrix
Author(s) -
Sun Aili,
Zheng Jianbin,
Sheng Qinglin
Publication year - 2011
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201190136
Subject(s) - chemistry , hydrogen peroxide , nanocomposite , biosensor , detection limit , electrochemistry , selectivity , carbon nanotube , nuclear chemistry , redox , matrix (chemical analysis) , electrode , inorganic chemistry , analytical chemistry (journal) , nanotechnology , catalysis , chromatography , organic chemistry , materials science , biochemistry
A novel nanocomposite designed by the assembly of the positively charged poly(diallyldimethylammonium chloride) protected gold nanoparticles (PDDA‐GNPs), and the negatively charged multi‐walled carbon nanotubes (MWCNTs) on ITO electrode via electrostatic interaction, was used as a supporting matrix for immobilizing hemoglobin (Hb) to develop a high‐performance hydrogen peroxide (H 2 O 2 ) biosensor. The cyclic voltammetrys of immobilized Hb showed a pair of well‐defined and quasi‐reversible redox peaks with the formal potential of ‐0.205V (vs. SCE) and the peak‐to‐peak potential separation of 44 mV at a scan rate of 100 mV×s −1 in 0.1 mol×L −1 pH 7.0 PBS. Under the optimized experimental conditions, a linearity range for determination of H 2 O 2 was from 2.0 × 10 −6 to 5.2 × 10 −4 mol×L −1 with a correlation coefficient of 0.9994 (n = 37) and a detection limit of 8.4 × 10 −7 mol×L −1 . The biosensor displayed excellent electrochemical and electrocatalytic response to the reduction of H 2 O 2 , high sensitivity, long‐term stability, good bioactivity and selectivity.