Premium
Electrodeposition CaCO 3 Nanoparticles‐chitosan Composite Film on Carbon Ionic Liquid Electrode as a Platform for Hemoglobin Electrochemical Biosensor
Author(s) -
Zhao HongYe,
Zheng JianBin,
Sheng QingLin
Publication year - 2011
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201190036
Subject(s) - chemistry , ionic liquid , electrochemistry , chitosan , electrode , biosensor , electron transfer , dielectric spectroscopy , charge transfer coefficient , redox , electrocatalyst , nanoparticle , detection limit , nuclear chemistry , reaction rate constant , analytical chemistry (journal) , nanotechnology , inorganic chemistry , kinetics , cyclic voltammetry , catalysis , chromatography , organic chemistry , materials science , biochemistry , physics , quantum mechanics
By one‐step co‐electrodeposition CaCO 3 nanoparticles‐chitosan composite film on carbon ionic liquid electrode (CILE), and then by spreading the composition of hemoglobin (Hb) and chitosan on the nanoCaCO 3 ‐chi/CILE, a Hb‐chi/nanoCaCO 3 ‐chi/CILE was fabricated and the direct electrochemistry and electrocatalysis of Hb at the electrode was investigated. The electrochemical impedance spectroscopy of the modified electrode showed the electron transfer resistance was 1166 Ω. Investigation results of cyclic voltammetrys showed a pair of well‐defined and quasireversible redox peak of Hb with the formal potentials of ‐0.295 V ( vs . SCE) in 0.1 mol·L ‐1 pH 7.0 PBS; the response time of the reduction peak currents of Hb was lower than 3s; a linear range for determination of H 2 O 2 was from 5.0 μmol·L ‐1 to 1.3 mmol·L ‐1 with a detection limit of 1.6 μmol·L ‐1 (S/N = 3) and a sensitivity of 0.16 A·M ‐1 ·cm ‐2 ; the electron transfer rate constant and the apparent Michaelis‐Menten constant of Hb were 1.98 s ‐1 and 0.81 mmol·L ‐1 , respectively. As a result, the case of the one‐step co‐electrodeposition and the promising feature of biocomposite could serve as a versatile platform for the fabrication of electrochemical biosensors.