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Extraction of Substance P by Functionalized Iron Oxide Nanoparticles for Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometric Analysis
Author(s) -
Chang YenHsiang,
Chen ZhiMeng,
Peng HsienYu,
Tsai YuanJen,
Lin TzerBin,
Chen GinDen,
Wang C. R. Chris,
Change Sarah Y.
Publication year - 2011
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201190006
Subject(s) - chemistry , desorption , detection limit , adsorption , nanoparticle , iron oxide nanoparticles , chromatography , matrix assisted laser desorption/ionization , extraction (chemistry) , aqueous solution , matrix (chemical analysis) , mass spectrometry , analytical chemistry (journal) , iron oxide , organic chemistry , nanotechnology , materials science
Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix‐assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP‐adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7%, respectively.