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Direct Electrochemistry of Glucose Oxidase Immobilized on Titanium Carbide‐Au Nanoparticles‐Fullerene C 60 Composite Film and Its Biosensing for Glucose
Author(s) -
Wang Minzhi,
Zheng Jianbin,
Wang Lu,
Dong Sheying
Publication year - 2012
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201100729
Subject(s) - chemistry , biosensor , glucose oxidase , cyclic voltammetry , electrochemistry , detection limit , electron transfer , nuclear chemistry , charge transfer coefficient , glassy carbon , redox , nanoparticle , electrode , analytical chemistry (journal) , inorganic chemistry , nanotechnology , chromatography , organic chemistry , materials science , biochemistry
The direct electrochemistry of glucose oxidase (GOD) immobilized on the designed titanium carbide‐Au nanoparticles‐fullerene C 60 composite film modified glassy carbon electrode (TiC‐AuNPs‐C 60 /GCE) and its biosensing for glucose were investigated. UV‐visible and Fourier‐transform infrared spectra of the resulting GOD/TiC‐AuNPs‐C 60 composite film suggested that the immobilized GOD retained its original structure. The direct electron transfer behaviors of immobilized GOD at the GOD/TiC‐AuNPs‐C 60 /GCE were investigated by cyclic voltammetry in which a pair of well‐defined, quasi‐reversible redox peaks with the formal potential ( E 0′ ) of ‐0.484 V ( vs. SCE) in phosphate buffer solution (0.05 M, pH 7.0) at the scan rate of 100 mV·s −1 were obtained. The proposed GOD modified electrode exhibited an excellent electrocatalytic activity to the reduction of glucose, and the currents of glucose reduction peak were linearly related to glucose concentration in a wider linearity range from 5.0 × 10 −6 to 1.6 × 10 −4 M with a correlation coefficient of 0.9965 and a detection limit of 2.0 × 10 −6 M ( S/N = 3). The sensitivity and the apparent Michaelis‐Menten constant ( K M app ) were determined to be 149.3 μA·mM −1 ·cm −2 and 6.2 × 10 −5 M, respectively. Thus, the protocol will have potential application in studying the electron transfer of enzyme and the design of novel electrochemical biosensors.