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Electrochemical Determination of Tyrosine in the Presence of Dopamine and Uric Acid at the Surface of Gold Nanoparticles Modified Carbon Paste Electrode
Author(s) -
Ghoreishi S. M.,
Behpour M.,
Jafari N.,
Golestaneh M.
Publication year - 2012
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201100654
Subject(s) - chemistry , chronoamperometry , differential pulse voltammetry , cyclic voltammetry , carbon paste electrode , electrode , electrochemistry , detection limit , buffer solution , colloidal gold , uric acid , calibration curve , nuclear chemistry , analytical chemistry (journal) , inorganic chemistry , chromatography , nanoparticle , nanotechnology , biochemistry , materials science
Abstract A gold nanoparticles modified carbon paste electrode (GN‐CPE) was used as a highly sensitive electrochemical sensor for determination of tyrosine (Tyr), dopamine (DA) and uric acid (UA) in phosphate buffer solution (PBS). The study and measurements were carried out by using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry methods. In DPV, the GN‐CPE could separate the oxidation peak potentials of DA and UA present in the same solution, though at the unmodified CPE the peak potentials were indistinguishable. The prepared electrode showed voltammetric responses with high sensitivity and selectivity for Tyr, DA and UA in optimal conditions, which makes it very suitable for simultaneous determination of these compounds. The calibration curves for Try, DA and UA were linear for the concentrations of each species. The proposed voltammetric approach was also applied to the determination of Tyr concentration in human serum as a real sample.