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Isolation and Purification of Flavonoids from Ziziphus jujuba by High‐Speed Counter‐Current Chromatography
Author(s) -
Bai HeLong,
Wang Jing,
Liu ChunMing,
Li Li
Publication year - 2010
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201000150
Subject(s) - ziziphus jujuba , chemistry , chromatography , countercurrent chromatography , electrospray ionization , chromatography detector , mass spectrometry , ziziphus , high performance liquid chromatography , botany , biology
High‐Speed Counter‐Current Chromatography (HSCCC) coupled with Diode array detector (DAD) was successfully applied to the isolation and purification of three flavonoids from Ziziphus jujuba . The solvent system was composed of ethyl acetate‐n‐butanol‐water (3:2:5, v/v/v). The purity of compounds 1 , 2 and 3 was above 90.0% by HPLC‐DAD and the structures of these compounds were identified by electrospray ionization tandem mass spectrometry (ESI‐MS n ) as spinosin, 6′″‐sinapoylspinosin, 6′″‐feruloylspinosin.
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