Premium
The Use Ionic Liquid as the Eluent Additive for HPLC Separation of Ionic Dyes
Author(s) -
Chen HsiuLiang,
Wei GuorTzo
Publication year - 2010
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201000116
Subject(s) - chemistry , ionic liquid , high performance liquid chromatography , chromatography , column chromatography , ionic bonding , alkyl , acetonitrile , reversed phase chromatography , organic chemistry , ion , catalysis
Reversed‐phase HPLC separation of ionic dyes (methylene blue, neutral red, methyl orange, and acid orange) with 1‐alkyl‐3‐methylimidazolium ionic liquids as the eluent additives was studied. The addition of ionic liquid to the eluent affects the resolution, peak shape and reproducibility of the retention of ionic solutes. The use of ionic liquids as the additives facilitates the separation of ionic dyes with a reversed‐phase HPLC. Baseline‐resolved separation of these dyes was achieved in 12 minutes when the mobile phase of acetonitrile‐water (28:72,v/v) contained 0.3‐2.0% (v/v) [emim][BF 4 ]. The detection limits for the four dyes ranged from 4.45 to 10.0 μg/L for an injection volume of 20 μL. Apparently, ionic liquid is an effective mobile phase additive for the separation of ionic dyes in reversed‐phase HPLC separation.