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Simultaneous Quantitation of Captopril and NSAID's in API, Dosage Formulations and Human Serum by RP‐HPLC
Author(s) -
Sultajma,
Arayne M. Saeed,
Naveed Safila
Publication year - 2010
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201000010
Subject(s) - chemistry , mefenamic acid , chromatography , captopril , flurbiprofen , diclofenac sodium , detection limit , high performance liquid chromatography , dosage form , ibuprofen , diclofenac , phosphoric acid , pharmacology , medicine , biochemistry , organic chemistry , blood pressure , radiology
Abstract An accurate, sensitive and least time consuming reverse phase high performance liquid chromatographic (RP‐HPLC) method for the estimation of captopril in the presence of non steroidal anti‐inflammatory drugs in formulation and human serum has been developed and validated. Chromatographic separation was conducted on prepacked Purospher star C18 (5 μm, 25 × 0.46 cm) column at room temperature using methanol:water (80:20 v/v) as a mobile phase, pH adjusted at 2.8 with o ‐phosphoric acid and at a flow rate of 1.0 mL min −1 , while UV detection was performed at 227 nm. The limit of detection and quantification for captopril were 1 and 0.35 ng mL −1 , while that for (NSAID's) i.e. flurbiprofen, ibuprofen, diclofenac sodium and mefenamic acid LOD were 0.2, 1, 2 and 0.4 ng mL −1 respectively and LOQ were 0.9, 2.9, 8 and 1 ng mL −1 Analytical recovery was > 98.1%. The method used for the quantitative analysis of commonly administered non steroidal anti‐inflammatory drugs (NSAID's) i.e. ibuprofen, flurbiprofen, diclofenac sodium and mefenamic acid alone or in combination with captopril from API (active pharmaceutical ingredients), dosage formulations and in human serum. The established method is rapid (RT < 12 min), accurate (recovery > 98.1%), selective (no interference of excepients and other commonly used drugs and food) and sensitive (LOQ 3.5 ng mL ;‐1 ) and reproducible (SD ± 0.003).