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A Simple and Cheap Device for Colorimetric Determination of Serum Iron
Author(s) -
Sorouraddin M. H.,
Saadati M.,
Samadi A.
Publication year - 2010
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201000009
Subject(s) - colorimeter , chemistry , multimeter , colorimetry , calibration curve , serum iron , chromatography , standard curve , analytical chemistry (journal) , standard solution , detection limit , hemoglobin , biochemistry , optics , physics , voltage , quantum mechanics
A simple and cheap device for colorimetric determination of serum iron and TIBC (total iron‐binding capacity) was devised. The proposed device employs an LED as a light source and a common light dependent resistor (LDR) as a detector. This device functions on the basis of the light intensity received by LDR, connected to a digit multimeter, yielding resistance readings. The serum, standard, and blank solutions were prepared according to the kits instructions and introduced into the colorimeter with a disposable syringe. The iron content of the serum was calculated from the resistance difference of serum and standard solutions. The precision of the method was assessed with two commercially available serum‐based chemistry controls. The values obtained with the proposed device indicated that the serum iron concentrations correlated well with the values obtained with the commercial automated analyzer. The calibration graph was linear for iron concentrations up to 90 μmol/L (500 μg/dL). The proposed fabricated colorimeter is considerable cheaper, requires smaller sample volumes, and is suitable for serum iron assay.