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Layered Zirconium(IV) Aminoethylphosphonate Intercalated with Horseradish Peroxidase for an Amperometric Biosensor
Author(s) -
Liu ChangHua,
Guo XiaoLi,
Yuan Ruo
Publication year - 2009
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200900145
Subject(s) - horseradish peroxidase , biosensor , chemistry , amperometry , scanning electron microscope , detection limit , hydrogen peroxide , electrode , nuclear chemistry , zirconium , chromatography , inorganic chemistry , electrochemistry , organic chemistry , materials science , enzyme , biochemistry , composite material
Layered zirconium(IV) aminoethylphosphonate (ZrAEP) have been used as matrices for immobilization of horseradish peroxidase (HRP) to fabricate enzyme electrode for an amperometric biosensor. The biocompatible HRP–ZrAEP films were fabricated on gold electrode surface by electro‐co‐deposition method. The morphology of the HRP–ZrAEP composite was characterized by scanning electron microscopy (SEM). UV–vis spectroscopy indicated that the intercalated HRP retained its native structure after incorporation in the ZrAEP. The immobilized HRP at the HRP–ZrAEP films exhibited good electro catalytic responses to the reduction of hydrogen peroxide. The response time of the biosensor was less than 3 s, and the linear range is from 2.5 × 10 −6 to 3.22 × 10 −3 M, with a detection limit of 7.0 × 10 −7 M (S/N = 3). The Michaelis–Menten constant ( K app M ) value is estimated to be 2.21 mM. In addition, the obtained biosensor possesses high sensitivity, good stability and reproducibility.