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Determination of Nitrofuran Residues in Tilapia Tissue by Enzyme‐Linked Immunosorbent Assay and Confirmation by Liquid Chromatography Tandem Mass Spectrometric Detection
Author(s) -
Tsai ChungWei,
Hsu ChiHsin,
Wang WeiHsien
Publication year - 2009
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200900086
Subject(s) - chemistry , furazolidone , chromatography , nitrofuran , derivatization , detection limit , liquid chromatography–mass spectrometry , metabolite , tandem mass spectrometry , tilapia , mass spectrometry , fish <actinopterygii> , antibiotics , biochemistry , genetics , fishery , biology
Furazolidone is a broad‐spectrum antibiotic that is frequently used in aquaculture on account of its excellent antibacterial properties. In this study, both the enzyme‐linked immunosorbent assay (ELISA) and high‐performance liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) methods were used to analyze the content of residual 3‐amino‐2‐oxazolidinone (AOZ), a metabolite of furazolidone in Tilapia tissue. Homogenized fish samples were spiked with various amounts of AOZ, and following combined acid‐hydrolysis and derivatization of the homogenized tissue with 2‐NBA (2‐nitrobenzaldehyde), sample clean‐up was performed and the derived 2‐nitrophenylmethylene‐3‐amino‐2‐oxazolidinone (NPAOZ) was analyzed. Using the LC‐MS/MS method, a linear correlation between measured concentration Y and spiked concentration × was observed: Y = 0.4518X − 0.0166, R 2 = 0.9972. The linear equation for the ELISA method was Y = 0.9322X + 0.5168, R 2 = 0.9066. These results demonstrated that the ELISA method might overestimate the residual AOZ content at low concentrations. The detection limit and recovery of the known addition were 0.05 μg kg −1a and 108% for the LC‐MS/MS method and 0.31 μg kg −1 and 305% for the ELISA method, respectively.