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TH‐11, a Streptomyces sp. Strain that Degrades Poly(3‐Hydroxybutyrate) and Poly(Ethylene Succinate)
Author(s) -
Hoang KimChi,
Lee ChungYi,
Lai YiChi,
Liau ChunYi
Publication year - 2008
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200800181
Subject(s) - chemistry , strain (injury) , polymer , agar , streptomyces , gelatin , ethylene , degradation (telecommunications) , agar plate , nuclear chemistry , polymer chemistry , organic chemistry , bacteria , medicine , telecommunications , biology , computer science , genetics , catalysis
Abstract TH‐11, a bacterial strain with strong depolymerase activity that breaks down aliphatic esters such as poly(3‐hydroxybutyrate) (PHB) and poly(ethylene succinate) (PES) was isolated from a soil sample collected from the sediment of Tou‐Chain River, Taiwan, R.O.C. It was phenotypically and genetically characterized to be a Streptomyces strain. The degradation of PHB and PES were tested both using emulsified polymers in solid agar and thin polymer films in liquid culture media. The degradations were measured by clear‐zone formation on solid agar plates, or direct weight measurements and electromicroscope inspection of the incubated polymer films in the liquid culture. The depolymerase activities can be detected in the cell‐free preparation of the culture medium, and can be enhanced by gelatin.