Premium
Extractive Spectrophotometric Methods for Determination of Clarithromycin in Pharmaceutical Formulations Using Bromothymol Blue and Cresol Red
Author(s) -
Shah Jasmin,
Jan M. Rasul,
Manzoor Suraya
Publication year - 2008
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200800162
Subject(s) - bromothymol blue , chemistry , absorbance , molar absorptivity , clarithromycin , chloroform , chromatography , aqueous solution , spectrophotometry , ion association , nuclear chemistry , organic chemistry , biochemistry , physics , optics , antibiotics
Two simple and sensitive extractive spectrophotometric methods have been described for the analysis of clarithromycin in pure form and in pharmaceutical formulations. The methods involved formation of yellow colored chloroform extractable ion‐association complexes of clarithromycin with bromothymol blue (BTB) and cresol red (CR) in buffered aqueous solution at pH 4. The extracted complexes showed maximum absorbance at 410 and 415 nm for BTB and CR, respectively. Beer's law is obeyed in the concentration ranges 0.1–20 μg mL −1 and 2.0–20 μg mL −1 of clarithromycin with molar absorptivity of 2.01 × 10 4 and 4.378 × 10 3 for BTB and CR, respectively. The composition ratio of the ion‐association complex was clarithromycin: BTB and CR = 1:1 as established by Job's method. The methods have been applied to the determination of drug in commercial formulations. The results of analysis were validated statistically and through recovery studies.