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Direct Electron Transfer of Hemoglobin on a Carbon Ionic Liquid Electrode with TiO 2 Nanopatricle as Enhancer
Author(s) -
Sun Wei,
Li XiaoQing,
Jiao Kui
Publication year - 2008
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200800157
Subject(s) - chemistry , hexafluorophosphate , ionic liquid , electron transfer , redox , electrochemistry , electrode , charge transfer coefficient , adsorption , inorganic chemistry , carbon paste electrode , standard electrode potential , analytical chemistry (journal) , cyclic voltammetry , catalysis , organic chemistry
Abstract Room temperature ionic liquids (RTILs) N‐butylpyridinium hexafluorophosphate (BPPF 6 ) modified carbon paste electrode (CILE) was fabricated and applied to adsorb the hemoglobin (Hb) and TiO 2 nanoparticles on the electrode surface step by step to form a Hb modified electrode noted as TiO 2 /Hb/CILE. UV‐Vis and FT‐IR spectra showed that Hb in the film retained its native conformations. Cyclic voltammetric experiments indicated that a pair of well‐defined quasi‐reversible redox peaks appeared with the formal potential (E 0′ ) located at −0.251 V (vs. SCE) at pH 7.0 phosphate buffer solution (PBS), which was the characteristic of heme Fe(III)/Fe(II) redox couples. Electrochemical parameters of the Hb in the film such as the electron transfer coefficient (α), the electron transfer number (n) and the standard electron transfer rate constant ( k s ) were estimated as 0.469, 0.87 and 0.635 s −1 , respectively.