Premium
Kinetic Spectrophotometric Determination of Ascorbic Acid in Pharmaceutical Samples by Oxidation of Ponceau 4R by Hydrogen Peroxide
Author(s) -
Grahovac Zora M.,
Mitić Snežana S.,
Pecev Todor G.,
Pecev Emilija T.,
Pavlović Aleksandra N.
Publication year - 2008
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200800021
Subject(s) - chemistry , ascorbic acid , hydrogen peroxide , detection limit , buffer solution , catalysis , calibration curve , inorganic chemistry , nuclear chemistry , chromatography , organic chemistry , food science
Abstract A new sensitive and simple kinetic method is developed for determination of traces of ascorbic acid based on its activated effect on oxidation of trisodium‐2‐hydroxy‐1‐(4‐sulphonato‐1‐naphthylazo)naphthalene‐6,8‐disulphonato (red artificial color Ponceau 4R) by hydrogen peroxide, in the presence of Cu(II) as catalyst, in borate buffer. The reaction is followed spectrophotometrically by tracing the oxidation product at 478.4 nm within 1 min after addition of H 2 O 2 . The optimum reaction conditions are: borate buffer (pH = 11.00), Ponceau 4R (9.6·10 −6 mol/L), H 2 O 2 (2·10 −2 mol/L), Cu(II) (8·10 −7 mol/L) at 22 °C. Following this procedure, ascorbic acid can be determined with a linear calibration graph up to 1.76 ng/mL and a detection limit of 0.28, based on 3S criterion. The relative error ranges between 6.77‐1.66% for the concentration interval of ascorbic acid 1.76‐17.61 ng/mL. The effects of certain foreign ions upon the reaction rate were determined for an assessment of the selectivity of the method. The method was applied for determination of ascorbic acid in pharmaceutical samples, and spectrophotometric method was used like an comparative method.