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Kinetic Spectrophotometric Determination of Metoprolol Tartrate in Commercial Dosage Forms
Author(s) -
Rahman Nafisur,
Haque Sk Manirul,
Azmi Syed Najmul Hejaz
Publication year - 2007
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200700213
Subject(s) - metoprolol tartrate , chemistry , tartrate , absorbance , calibration curve , chromatography , dosage form , linear regression , calibration , metoprolol , detection limit , analytical chemistry (journal) , statistics , mathematics , inorganic chemistry , medicine , cardiology
A kinetic spectrophotometric method has been described for the determination of metoprolol tartrate in commercial dosage forms. The procedure is based on the reaction of the drug with 1‐chloro‐2, 4‐dinitrobenzene (CDNB) in dimethylsulfoxide (DMSO) at 100 ± 1 °C. The reaction is investigated by measuring the change in absorbance with time at 420 nm. Fixed‐time (ΔA) and equilibrium methods are chosen for obtaining the calibration curves. Both calibration curves were found to be linear over the concentration range of 5‐60 μg mL −1 . The regression analysis of calibration data resulted in the linear regression equations of ΔA = −1.608 × 10 −4 + 3.96 × 10 −3 C and A = 7.31 × 10 −4 + 1.90 × 10 −2 C for fixed time (ΔA) and equilibrium methods, respectively. The limit of detection (LOD) for fixed time and equilibrium methods are 1.16 and 0.415 μg mL −1 , respectively. The method has been successfully applied to the quantitation of metoprolol tartrate in commercial dosage forms. Statistical comparison of the results shows that there is no significant difference between the proposed methods and El‐Ries's spectrophotometric method.