Premium
PVC‐Based on Thiopyrilium Derivatives Membrane Electrodes for Determination of Histamine
Author(s) -
Javanbakht Mehran,
Mohammadi Ali,
Ganjali Mohammad Reza,
Norouzi Parviz,
Faridbod Farnoush,
Pirelahi Hooshang
Publication year - 2007
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200700211
Subject(s) - chemistry , histamine , electrode , selectivity , ionophore , membrane , perchlorate , cationic polymerization , detection limit , glass electrode , ion selective electrode , inorganic chemistry , chromatography , organic chemistry , electrochemistry , reference electrode , ion , biochemistry , catalysis , medicine
This study describes novel histamine‐selective electrodes, having their basis on thiopyrilium (TP) derivatives as suitable ionophores. The electrodes were prepared by incorporating the TP derivatives into plasticized poly(vinyl chloride) (PVC) membranes. These electrodes demonstrate high selectivity as far as the histamine response is considered, as compared with many common inorganic anions and other kinds of amino acids. The influence of membrane composition, pH and the effect of lipophilic cationic and anionic additives on the response characteristics of the electrode were investigated. The resulting sensor based on 2,6‐bis(4‐dimethyl amino phenyl)‐4‐phenyl thiopyrilium perchlorate (TP4) responds to histamine in a wide concentration range from 5.0 × 10 −6 to 1.0 × 10 −1 M with a slope of 54.8 ± 0.6 mV decade −1 and detection limit of 3.0 μ mol L −1 (∼0.3 ppm). The electrode illustrates fast response time and good long‐term stability (more than 2 months). The ability to design histamine‐selective electrodes based on new thiopyrilium derivatives and both to alter selectivity and improve the response characteristics through structural changes to the charged ionophore, has been investigated. The prepared electrode was used for the determination of histamine in a synthetic human serum sample. Consequently, satisfactory recovery results were obtained over a wide concentration range of histamine.