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Adsorptive Stripping Differential Pulse Voltammetry for Determination of Trace Amounts of Noscapine in Human Plasma
Author(s) -
Niazi Ali
Publication year - 2007
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200700170
Subject(s) - noscapine , chemistry , detection limit , analytical chemistry (journal) , chromatography , voltammetry , hanging mercury drop electrode , adsorptive stripping voltammetry , differential pulse voltammetry , calibration curve , electrode , cyclic voltammetry , electrochemistry , stereochemistry , alkaloid
A new adsorptive anodic differential pulse stripping voltammetry method for the direct determination of noscapine at trace levels in human plasma of addicts is proposed. The procedure involves an adsorptive accumulation of noscapine on a hanging mercury drop electrode (HMDE), followed by oxidation of adsorbed noscapine by voltammetry scan using differential pulse modulation. The optimum conditions for the analysis of noscapine are pH = 8.5 using Britton‐Robinson (B‐R) buffer, accumulation potential of −100 mV (vs. Ag/AgCl), and accumulation time of 150 s. The peak current is proportional to the concentration of noscapine, and a linear calibration graph is obtained at 0.015–2.75 μg mL −1 . A relative standard deviation of 1.28% (n = 5) was obtained, and the limit of detection was 7 ng mL −1 . The capability of the method for the analysis of real samples was evaluated by determination of noscapine in spiked human plasma and addicts, human plasma with satisfactory results.