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Application of Boron as the Chromogenic Agent in Spectrophotometry I. A Novel Fading Spectrophotometric Method for the Determination of Aminomethylbenzoic Acid with Boric Acid
Author(s) -
Li QuanMin,
Zhang TianTian
Publication year - 2007
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200700141
Subject(s) - chemistry , chromogenic , boric acid , absorbance , detection limit , spectrophotometry , boron , chromatography , correlation coefficient , stoichiometry , linear range , ion association , analytical chemistry (journal) , ion , organic chemistry , statistics , mathematics
It is the first time that boron has been used as a chromogenic agent to determine aminomethylbenzoic acid (PAMBA) by the fading spectrophotometric method in this paper. The study indicates that at pH 10.00 the absorbance of PAMBA decreases when boric acid (BA) is added to the solution. A simple, rapid, sensitive and reliable novel method based on the product of PAMBA and BA is obtained. The stoichiometric ratio of the product is 1:2. Beer's law is obeyed in the range of PAMBA concentrations of 0.13 ˜ 38.40 μg/mL at a wavelength of 242 nm (ε 242 is 8.1 × 10 4 L/mol/cm). The equation of linear regression is A = ‐0.06635 – 0.02914C (× 10 −5 M), with a linear correlation coefficient of 0.9974. The detection limit is 0.12 μg/mL and RSD 0.11%. The method is successfully applied to the determination of PAMBA in pharmaceutical samples, and average recoveries from pharmaceutical samples and urine samples were between 98.4 ˜ 102.5%. The other components in these samples do not interfere with the determination by the proposed method.