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Studies on the Spectra of 4,5‐Dibromofluorescein with Proteins and its Analytical Application
Author(s) -
Cheng DingXi,
Wang ShaoHua,
Liu WenJie
Publication year - 2006
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200600093
Subject(s) - chemistry , coomassie brilliant blue , detection limit , quenching (fluorescence) , fluorescence , analytical chemistry (journal) , chromatography , linear range , optics , medicine , staining , physics , pathology
4,5‐Dibromofluorescein(R) binding to proteins causes a decrease in the fluorescence maximum of R at 530 nm; the intensity of fluorescence quenching is directly proportional to protein content, Based on this, a new fast and simple fluorescence quenching method for the determination of proteins was developed. Under experimental conditions, the linear range of this assay is 0˜15 mg/L and the detection limit is 40 μg/L. The method has been applied to the analysis of human serum samples and gave values close to those obtained by the Coomassie brilliant blue G‐250 (CBB G‐250) method, which indicated that the method is not only high in sensitivity but also reliable.