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Synthesis of Activity Probes for β‐Xylosidase
Author(s) -
Lo LeeChiang,
Chu ChiYuan,
Lin FangAn,
Pan YenRu,
Li YawKuen,
Lin JingJer
Publication year - 2006
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200600063
Subject(s) - chemistry , linker , biotinylation , biotin , biochemistry , pulp (tooth) , hydrolase , combinatorial chemistry , computational biology , enzyme , medicine , pathology , computer science , biology , operating system
Due to their industrial interest and significance, developing a rapid method for screening β‐xylosidase activities would certainly facilitate the research and improve production in xylan‐related industries especially in the pulp and paper industries. Here we report the synthesis of two activity probes, LCL‐ 6X and ‐ 12X , for β‐xylosidase. They both carry a β‐xylopyranosyl recognition head, which is linked to a latent trapping device consisting of a 2‐fluoromethylphenoxyl group and a biotin reporter group. They differ only in the length of their hydrophobic linker. A model study using a β‐xylosidase from T. koningii demonstrates that both probes could successfully label the target hydrolase and give rise to biotinylated proteins. They could thus potentially become a powerful tool in screening β‐xylosidase from microbial sources.