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A Linear Sweep Voltammetric Determination of DNA with Methyl Violet
Author(s) -
Sun Wei,
Shang Zhimei,
Li Qingjun,
Jiao Kui
Publication year - 2005
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200500183
Subject(s) - chemistry , buffer solution , detection limit , linear sweep voltammetry , analytical chemistry (journal) , aqueous solution , stoichiometry , peak current , electrochemistry , linear range , linear relationship , electrode , chromatography , cyclic voltammetry , statistics , mathematics
A new quantitative method for the determination of DNA in aqueous solution based on the interaction of methyl violet (MV) with fsDNA by linear sweep voltammetric technique is proposed in this paper. The interaction of MV with fsDNA caused the decrease of the reductive peak current of MV at −0.650 V (vs. SCE) on a mercury electrode in pH 1.5 Britton‐Robinson (B‐R) buffer solution. Factors including the acidity of the buffer, the reaction time, the optimal concentration of MV, the reaction temperature, the influences of the foreign substances, etc. were all carefully investigated. Under the optimal conditions, the decrease of the reductive peak current of MV was proportional to the concentration of fsDNA in the linear range of 0.90∼20.0 mg/L and the detection limit for fsDNA was 0.25 mg/L with the RSD of 4.00%. Synthetic samples were determined with satisfactorily results. The stoichiometry of MV with DNA was calculated by voltammetric data and the results showed that the binding ratio of DNA:MV was 1:2 with the binding constant β as 7.36 × 10 8 .