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Investigation of the Photodecomposition of Hemin and Trace Amount Determination of Hemin with Flow Injection Chemiluminescence Method
Author(s) -
Han SuQin,
Liu ErBao,
Li Hua
Publication year - 2005
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200500060
Subject(s) - hemin , chemistry , chemiluminescence , detection limit , linear range , decomposition , aqueous solution , chromatography , nuclear chemistry , organic chemistry , heme , enzyme
Using the hemin‐H 2 O 2 ‐Na 2 CO 3 ‐NaOH chemiluminescence (CL) system, the study on the photodecomposition behavior of hemin under ultraviolet light and solar light were carried out and the determination of hemin was developed coupled with simple flow injection technique. The results showed that the decomposition reaction of hemin in different light irradiations corresponded with a first‐order reaction. And then the determination of hemin was completed by the CL emission from the reaction of hemin with H 2 O 2 in aqueous carbonate. The linear range was 2.2 × 10 −10 to 6.88 × 10 −7 M and the detection limit was 2.2 × 10 −11 M (S/N = 3). The relative standard deviation (RSD) was 2.82% for ten independent detections of 1.72 × 10 −8 M hemin. As a preliminary application, the proposed method was successfully applied for the analysis of hemin in pharmaceutical formulations and animal blood with a recovery of 96˜108%. A possible CL mechanism of the present system was discussed, and free radicals were suggested to be involved in this reaction.