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DNA Hydrolysis Catalyzed by Tris(diisopropyl‐1,4,7‐triazacyclononanes)ethane Metal Complexes
Author(s) -
Cheng ChienChung,
Huang YanChen,
Liu MingChan
Publication year - 2004
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200400176
Subject(s) - chemistry , phosphodiester bond , hydrolysis , reactivity (psychology) , tris , steric effects , metal ions in aqueous solution , bond cleavage , metal , ligand (biochemistry) , cleavage (geology) , dna , catalysis , linker , medicinal chemistry , stereochemistry , organic chemistry , medicine , rna , biochemistry , alternative medicine , receptor , geotechnical engineering , pathology , fracture (geology) , computer science , gene , operating system , engineering
Abstract Novel trinuclear coordinated ligands were designed using three of 1,4,7‐triazacyclononane moieties with a tribromo linker. These ligands show greater capability and potency in association with various metal ions. Tris(diisopropyl‐1,4,7‐triazacyclononanes)ethane ( 2 ) associated with Cu 2+ and Ce 4+ ions shows better reactivity to achieve DNA hydrolysis at 37 °C at pH 8 for 5 hr. Using high resolution of polyacrylamide gel electrophoresis, DNA cleavage reaction in Tris‐HCl buffer causes a nicking site at the single‐stranded region. Alternatively, the reaction in HEPES buffer shows a random DNA cleavage included the double‐stranded region. Hydrolysis of phosphodiester bond mediated by trinuclear metal complex is capable of performing at the physiological temperature 37 °C, but it has a lower reactivity compared to mono‐ and di‐nuclear metal complexes. The result suggests that trinuclear metal complexes have a bulky environment to prevent from going toward the phosphodiester bond. The design of an efficient chemical hydrolyase will be a challenge in order to construct a coordinate ligand with the enhancement of hydrolytic activity and the reduction of the steric environment.