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Spectrofluorimetric Determination of Trace Amounts of Copper
Author(s) -
Liu JianNing,
Zhang Bing,
Wu BoWan,
Yu XinQiao
Publication year - 2004
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200400013
Subject(s) - chemistry , reagent , copper , fluorescence , trace amounts , chelation , inorganic chemistry , analytical chemistry (journal) , nuclear chemistry , chromatography , organic chemistry , medicine , physics , alternative medicine , pathology , quantum mechanics
A fluorescent reagent, 2,4‐dihydroxyacetophenone‐4‐hydroxybenzoylhydrazone (2,4‐DH‐AP‐4‐H‐BH) was studied on its fluorescent reaction with copper using the spectrofluorimetric method. In a water‐ethanol solution at pH 12.0, a chelating reaction occurs between copper and 2,4‐DH‐AP‐4‐H‐BH. The molar ratio of copper to the reagent is 1:2. This reaction causes characteristic changes of spectra of fluorescence with excitation and emission maxima at 342.4 and 419.2 nm, respectively. This method can quantitate the amount of copper in the reaction. It detects as little as 0.2 ppb of copper with a linear range between 0.2 to 120 ppb. It has been successfully applied to detect copper in mineral water, tea, and hair.