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Thin Layer Chromatography of Purines on Silica Gel G Impregnated with Transition Metal Ions; Assay of Caffeine and Theophylline in Pharmaceutical Formulations
Author(s) -
Singh D. K.,
Srivastava Bhavana,
Sahu Archana
Publication year - 2003
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200300145
Subject(s) - chemistry , purine metabolism , theophylline , silica gel , chromatography , thin layer chromatography , caffeine , metal ions in aqueous solution , purine , transition metal , metal , organic chemistry , catalysis , medicine , enzyme , endocrinology
This study was undertaken to develop thin layers of silica gel G impregnated with transition metal ions for separation, identification and estimation of purines. The influence of transition metal ions and eluting solvents on chromatographic behaviour (hR f ) has been studied. The method was applied for qualitative analysis of purines in the mixtures and quantitative analysis of purine bases in the mixture as well as in pharmaceutical formulations. The results were compared statistically with those obtained by official methods. The method is simple, reproducible, and accurate within 1.3 ± 0.6%.