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Simple Fourier Trans form(FT)‐IR and Reverse‐Phase HPLC Identification Methods of Commercial Ganoderma Products
Author(s) -
Chen DengHai,
Ju HueiYen,
Hseu KuoChong
Publication year - 2001
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200100178
Subject(s) - chemistry , ganoderma , mycelium , raw material , fourier transform infrared spectroscopy , high performance liquid chromatography , chromatography , phase (matter) , infrared spectroscopy , fourier transform , analytical chemistry (journal) , ganoderma lucidum , food science , botany , organic chemistry , chemical engineering , engineering , biology , mathematical analysis , mathematics
Ganoderma products could be classified into 3 types according to their raw materials, i.e. fruit bodies, cultured mycelia and the mixtures of both. Seven commercial samples and three mycelia products prepared in laboratory were analyzed by Fourier Trans form(FT)‐IR spectrophotometer. The fruit‐body products gave a weak absorption band at 890∼894 cm −1 and the mycelia show a weak to medium band at 774∼778 cm −1 on the FT‐IR spectra, respectively. These two bands were observed in the IR spectra of mixed‐type products. A modified sample pretreatment procedure for the reverse‐phase HPLC analysis was also investigated. The spectral patterns revealed that fruit bodies of the commercial samples were the species of G. tsugae .