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Polymer Coated Piezoelectric Quartz Crystal Protein Bio‐Sensors
Author(s) -
Hsu HuiPing,
Shih JengShong
Publication year - 2001
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200100029
Subject(s) - chemistry , chromatography , polyacrylamide , glutaraldehyde , polymer , organic chemistry , polymer chemistry
A polymer coated piezoelectric crystal detection system with a home‐made computer interface for signal acquisition and data processing was prepared as a liquid chromatographic detector for various proteins. Various polymers, e.g., polyvinyl aldehhyde (polyacrolein) (PVA), polyacrylamide/glutaldehyde (PAA/GA) and bio‐gel A, were used as coating materials on quartz crystals for adsorption of various protein molecules, e.g., catalase (CA), hemoglobin (Hb), α‐chymotrypsin (Ch), albumin (Ab). The frequency responses of the polyacrlein coated piezoelectric detector for various proteins were in the order: catalase> hemoglobin> α‐chymotrypsin > albumin. In contrast, the order of the frequency responses of bio‐gel A and polyacrylamide/glutaldehyde coated piezoelectric crystals for these proteins were: hemoglobin> catalase > α‐chymotrypsin ≥ albumin and hemoglobin > albumin > catalase. The polyacrolein coated piezoelectric crystal protein detector exhibited a good linear frequency response with a high sensitivity of about 2.5×10 3 Hz/(mg/mL) for catalase. In addition, bio‐gel A and polyacrylamide/glutaraldehyde coated crystals were sensitive to hemoglobin with sensitivities of about 4.5×10 3 Hz/(mg/mL) and 3.0×10 3 Hz/(mg/mL), respectively. Study of the interference of various organic molecules, e.g., alcohols, amines, ketones and carboxylic acids, in the detection of proteins with theses polymer coated crystals was also made. The polyacrolein coated crystal for proteins under went less interference from various organic molecules than bio‐gel A or polyacrylamide/glutaraldehyde coated crystals. Effects of coating load, concentration of proteins and flow rate of liquid chromatographic eluent were also investigated and discussed.