Premium
Structure Identification of Aporphine Alkaloids by On‐Line Coupling of HPLC‐NMR with Loop Storage
Author(s) -
Tseng LiHong,
Braumann Ulrich,
Godejohann Markus,
Lee ShoeiSheng,
Albert Klaus
Publication year - 2000
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200000168
Subject(s) - chemistry , high performance liquid chromatography , nuclear magnetic resonance spectroscopy , acetonitrile , elution , chromatography , analytical chemistry (journal) , gradient elution , coupling (piping) , resolution (logic) , proton nmr , nuclear magnetic resonance , stereochemistry , mechanical engineering , physics , artificial intelligence , computer science , engineering
The potential of coupling HPLC separation methodology to on‐line high resolution nuclear magnetic resonance (NMR) spectroscopy has been demonstrated with a mixture of nine aporphine alkaloids. A loop storage procedure after separation has facilitated the identification of closely eluted peaks (α = k 2 /k 1 = 1.01 and Δv = 0.37 min = 367 μL). These were collected off‐line and identified by a 1D 1 H NMR spectrum. For this purpose an automated procedure has been implemented which includes the use of shaped pulses, multiple solvents suppression and 13 C satellite suppression of acetonitrile.