Premium
Continuous Analysis of Urea in Blood by Immobilized Urease Using a Conductive Biosensor
Author(s) -
Shu HunChi,
Wu NingPing
Publication year - 2000
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200000137
Subject(s) - urease , chemistry , urea , dialysis , biosensor , chromatography , nuclear chemistry , biochemistry , surgery , medicine
Abstract Urea in blood from a patient undergoing dialysis was continuously monitored using immobilized urease reactor with a conductivity meter. A linear response of urea in the range between 5 to 50 mM with the throughput of 15 samples h −1 was obtained. Urea measured with this biosensor shows a good correlation with the chemical analysis method. A good operational stability for on‐line monitoring during dialysis and the promising storage stability of the urea reactor were found. At 4, and 25°C, no significant activity loss was detected in the urease reactor throughout the 50 weeks storage time. At 37°C, no activity decline was observed before 20 weeks. However, after 50 weeks storage, no activity in the reactor can be detected.