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Determination of Vitamin D 3 Metabolites Using High‐Performance Liquid Chromatography or Immunoaffinity Chromatography
Author(s) -
Shimada Kazutake,
Mitamura Kuniko,
Higashi Tatsuya
Publication year - 2000
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.200000037
Subject(s) - chemistry , chromatography , metabolite , high performance liquid chromatography , affinity chromatography , vitamin , vitamin d and neurology , quantitative analysis (chemistry) , active metabolite , biochemistry , medicine , enzyme
Our investigation of the analysis of vitamin D 3 metabolites has been reviewed. The development of high‐performance liquid chromatographic methods for the quantitative determination of 25‐hydroxyvitamin D 3 3‐sulfate and 25‐hydroxyvitamin D 3 , which are the major circulating metabolites of vitamin D 3 in human serum/plasma, has been described. The developed methods were applied to the determination of the correlation between the concentration of the sulfate and its genin in healthy subjects and patients with chronic renal failure. The development of immunoaffinity chromatography immobilizing the highly specific anti‐1,25‐dihydroxyvitamin D 3 antibody for the pretreatment of radioreceptor assay of 1,25‐dihydroxyvitamin D 3 , which is the active metabolite of vitamin D 3 , is also described.