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Systematic NMR Assignments of DNA Exchangeable Protons
Author(s) -
Tseng YuYu,
Chou ShanHo
Publication year - 1999
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199900096
Subject(s) - chemistry , dna , two dimensional nuclear magnetic resonance spectroscopy , proton , proton nmr , rna , crystallography , duplex (building) , zinc finger , fluorine 19 nmr , nuclear magnetic resonance spectroscopy , stereochemistry , biochemistry , physics , quantum mechanics , transcription factor , gene
Exchangeable proton information is of critical importance in the NMR structural studies of DNA/RNA oligomers and/or their complexes with protein. However, no systematic NMR assignment strategy about these protons has been reported until now, although similar studies about non‐exchangeable proton assignment have been well established in the past decade. We now report detailed NMR studies of a DNA duplex (d20‐mer) containing the recognition site (5′‐ATGAC‐3′) of GCN4 basic zinc finger protein. All exchangeable and non‐exchangeable protons except H5′/H5″ protons could be assigned from a single H 2 O/NOESY spectrum at 25°C. The assignment strategy reported in this paper shall be useful to NMR studies of unusual DNA/RNA structures and their complexes with peptides/proteins.