Premium
The Formation of Triple Stranded Helix of Oligodeoxyribonucleotides Containing 8‐Oxo‐2′‐deoxyadenosine
Author(s) -
Lin ShwuBin,
Wu ChiaMau,
Lin WeiChen,
Kan LouSing
Publication year - 1999
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199900095
Subject(s) - chemistry , cytosine , triple helix , deoxyadenosine , guanine , dna , stereochemistry , base pair , spermine , nucleotide , thymine , biochemistry , microbiology and biotechnology , gene , enzyme , biology
Triple stranded DNA helix (triplex) formation is limited by pH and the sequence of oligodeoxyribonucleotide. In this study, the 8‐oxo‐2′‐deoxyadenosine (8‐oxoA) can replace 2′‐deoxycytidine for forming a base triad with a base pair guanine and cytosine. The target for the triplex formation is a motif of a promotor (5′‐d‐TTAA, −3 – −12 of PKC α m‐RNA) of mouse protein kinase C α . The probes contain 20 bases with a common sequence 5′‐d‐TTAA. The portion may contain either 5‐methyl‐2′‐deoxycytidines, six 8‐oxoA's or both. Only the probe with a motif of six 8‐oxoA's can for triplex with the target in the presence of magnesium ion and spermine at neutral pH. Therefore, 8‐oxoA is useful for triplex formation under physiological conditions.