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Chemotaxonomy of Triterpenoid Pattern of HPLC of Ganoderma lucidum and Ganoderma tsugae
Author(s) -
Chen DengHai,
Shiou WenYue,
Wang KuenChern,
Huang ShuYing,
Shie YuTsan,
Tsai ChiouMan,
Shie JunFa,
Chen KuanDee
Publication year - 1999
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199900006
Subject(s) - chemistry , ganoderma , high performance liquid chromatography , ganoderma lucidum , triterpenoid , gradient elution , acetic acid , chromatography , botany , food science , biochemistry , stereochemistry , biology
Three strains of Ganoderma tsugae (CCRC36065, CCRC37034, CCRC37038) and three strains of Ganoderma lucidum (CCRC36021, CCRC37029, CCRC37033) were cultivated. Their triterpenoid patterns of the fruit body were analyzed by reverse phase HPLC using a gradient elution of acetonitrile/2% acetic acid (1/4 and 1/2). The triterpenoid patterns of G. tsugae and G. lucidum are different. But similar 2 and 3 dimensional patterns are obtained among three strains of G. tsugae. Different patterns are found among different strains of G. lucidum. Ganoderic acid A( 1 ), B( 2 ), C( 3 ) and D( 4 ) were isolated from the ethanol extract of G. tsugae.