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Aspartase‐Catalyzed Preparative Scale Synthesis of 15 N‐Aspartic Acid
Author(s) -
Sookkheo Boonyaras,
Phutakul Suree,
Chen ShuiTein,
Wang KungTsung
Publication year - 1998
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199800078
Subject(s) - chemistry , yield (engineering) , catalysis , enzyme , aspartic acid , chromatography , immobilized enzyme , ion exchange , ion exchange resin , combinatorial chemistry , nuclear chemistry , organic chemistry , ion , biochemistry , amino acid , materials science , metallurgy
A preparative scale synthesis of 15 N‐aspartic acid from fumarate and 15 NH 4 OH catalyzed by immobilized aspartase has been developed. Immobilization of aspartase in membrane enclosed enzyme immobilization (MEEI) facilitates the separation of the enzyme from product and makes the enzyme stable and durable for multiple usage. A simple isolation procedure in total yield of 62% using perfusion ion exchange chromatography renders the procedure more practical.