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Effect of Temperature in micro ‐HPLC Separation of PAHs under Isocratic Conditions Using Octadecyl and Alkylamide Phases
Author(s) -
GadzalaKopciuch Renata,
Buszewski Boguslaw
Publication year - 1998
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199800040
Subject(s) - chemistry , acetonitrile , high performance liquid chromatography , chromatography , phase (matter) , elution , gradient elution , chromatographic separation , amide , organic chemistry
The main difficulty in micro ‐HPLC separation is the manipulation with the composition of the mobile phase (degassing of solvents and a slow establishment of equilibrium) but the problem of elution can be solved by temperature optimalization. The effect of temperature in micro ‐HPLC separation of the 16 polycyclic aromatic hydrocarbons (PAHs) (mixture SRM 1647 of the US EPA) has been studied using conventional C 18 and new developed AP phase (an amide group localized in the hydrophobic ligands). All the investigations have been performed under isocratic conditions (binary hydroorganic mobile phase: acetonitrile/water). The results have shown that, in the case of AP phase, application of temperature gradient (from 298 to 303 K), enabled the attainment of complete 16 PAHs separation (especially of the first 4 solutes).