Premium
Isolation and Characterization of an Antibacterial Substance from Citrus Plant
Author(s) -
Ito Chihiro,
Fujiwara Kazuko,
Koizumi Meisaku,
Furukawa Hiroshi
Publication year - 1998
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199800015
Subject(s) - citrus canker , xanthomonas campestris , chemistry , xanthomonas citri , antibacterial activity , bacteria , rutaceae , isolation (microbiology) , rough lemon , botany , citrus paradisi , horticulture , traditional medicine , food science , microbiology and biotechnology , orange (colour) , biology , biochemistry , medicine , genetics , gene
Citrus bacterial canker caused by Xanthomomas campestris pv. citri (Hasse 1915) Dye 1978, a gram‐negative and rod‐shape bacteria, is one of the destructive diseases of Citrus. There is marked diversity in resistance to the disease among Citrus plants. Calamondin, Citrus madurensis Lour. (Shiki‐kitsu) is one of the highly resistant plants. 1 In resistant plants bacterial multiplication is restrained by the embedding of electron dense materials in intercellular spaces. 2 This suggests the presence of an antibacterial substance in resistant plants. The third author assayed the 85% ethanol extracts of Citrus leaves infected with bacterial canker for static activities to X. campestris. pv. citri. An active fraction was found in Calamondin. We further purified and characterized its ingredients on the basis of spectrometric analyses. Its structure was found to be identical with 3‐hydroxy‐5,6,7,8,3′,4′‐hexamethoxyflavone (1) isolated from Citrus natsudaidai Hayata. However, its antibacterial activity is first reported by us.