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Kinetic Study of S. griseus Aminopeptidase by Stopped‐Flow Fluorescence Energy Transfer
Author(s) -
Yu HsienCheng,
Lin WannYin
Publication year - 1998
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199800003
Subject(s) - chemistry , gibbs free energy , kinetic energy , reaction rate constant , hydrolysis , pronase , streptomyces griseus , activation energy , fluorescence , dissociation (chemistry) , analytical chemistry (journal) , kinetics , crystallography , enzyme , thermodynamics , chromatography , trypsin , organic chemistry , physics , quantum mechanics , bacteria , streptomyces , genetics , biology
Abstract The stopped‐flow fluorescence traces for the hydrolysis of Leu‐Ala‐DED catalyzed by Streptomyces griseus aminopeptidase isolated from pronase exhibits a two‐phase fluorescence change with comparable rates of formation and breakdown of the ES complex. We have developed a method for the determination of the individual rate constants from the stopped‐flow traces. The kinetic parameters for the enzyme‐catalyzed hydrolysis of Leu‐Ala‐DED at 25 °C and pH 8.0 are: k 1 = (1.6±0.1) × 10 5 M −1 s −1 , k −1 , = 0.089 ± 0.001 s −1 , and k 2 = 0.58 ± 0.01 s −1 . For the observed stopped‐flow traces, the steady state approximation is valid only within a very small region around the maximal ES concentration and a large proportion (> 20%) of the substrate has already been hydrolyzed during the pre‐steady state. For a very fast formation of ES complex, the steady state approximation is valid for almost the entire trace. The activation energies for each individual rate constant were determined to be 10.0 ± 0.7, 35 ± 5, and 21.2 ± 0.8 kcal mol −1 for k 1 , k −1 , and k 2 , respectively. Binding of E and S to form ES was accompanied by a decrease in Gibbs free energy, whereas a dramatic increase in free energy was observed for the conversion of ES to ES ≠ . The dissociation of ES to form E and S had a very large activation energy, but it was also accompanied by a large increase in entropy.