Premium
Direct Measurement of Enantiomeric Excess of Synthetic Tert‐Leucine with a Chiral‐Ligand‐Exchange Chromatography
Author(s) -
Wang ShwuHuey,
Kao HsinYi,
Chen ShuiTein,
Wang KungTsung
Publication year - 1995
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199500132
Subject(s) - chemistry , enantiomer , reagent , leucine , column chromatography , chromatography , enantiomeric excess , ligand (biochemistry) , chiral ligand , stereochemistry , organic chemistry , amino acid , catalysis , enantioselective synthesis , receptor , biochemistry
Racemic tert ‐leucine can be completely resolved by ligand exchange chromatography (LEC) using a Chiralpak WH column. The retention time of the tert ‐leucine was decreased by increasing concentration of Cu 2+ , or decreasing pH of the eluent whereas it was unaffected by varying the temperature of eluent. Optimal conditions for complete separation of the D.L‐ tert ‐leucine were found to be (Cu 2+ , 5.0 mM, pH 5.5, 25 °C). The enantiomeric excess of synthetic tert‐leucine, a chiral auxiliary reagent, was determined.