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Preparation and Characterization of Chemically Bound Tartrate‐Sillca Gel as Sorbent for Ligand Exchange Chromatography
Author(s) -
Chyueh SunDsong,
Liu ChuenYing
Publication year - 1994
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199400099
Subject(s) - chemistry , sorbent , metal ions in aqueous solution , inorganic chemistry , amide , ligand (biochemistry) , silica gel , metal , adsorption , nuclear chemistry , chromatography , organic chemistry , biochemistry , receptor
Tartrate‐based sorbents of two kinds for ligand‐exchange chromatography were prepared by covalent bonding of functional groups to silica gel through an amide bond. The structure and conversion of functional groups of the resin were confirmed with IR spectral and elemental analysis. The influence of pH on adsorption of metal ions to the resin was examined. The coordination behaviour of the sorbent was investigated by means of IR, EPR and electronic absorption spectroscopy. The results indicate that the affinity of metal ions toward the synthesized resins decreased in the order Cu(II) > Zn(II) > Cd(II). The conformation of the copper(II)‐sorbent complexes may be distorted tetragonal, whereas those of zinc(II) and cadmium(II) may be tetrahedral. These complexes between metal ion and sorbent were investigated as a stationary phase for enantiomeric separation of underivatized amino acids. Factors affecting the retention and the sample selectivity were examined. The interactions between sorbent metal complexes and analytes in this ternary system were also investigated.