z-logo
Premium
Comparison of Triple Quadrupole and Double Focusing Mass Spectrometers to Investigate Collision‐Induced Dissociation and Metastable Ions of Glycoconjugates
Author(s) -
Ju DarDer,
Li DingTzai,
Wei GuorJien,
Her GuorRong
Publication year - 1994
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199400044
Subject(s) - chemistry , ion , triple quadrupole mass spectrometer , mass spectrometry , mass spectrum , dissociation (chemistry) , collision induced dissociation , analytical chemistry (journal) , metastability , hybrid mass spectrometer , tandem mass spectrometry , selected reaction monitoring , chromatography , organic chemistry
Glycoconjugates, such as chromophore‐labeled disaccharides and permethylated glycosphingolipids (GSL) were used for comparison of triple quadrupole and double focusing mass spectrometers in analysis of product ions. A profound effect of collision energy was observed in the product ion spectra of ceramide ions (fragment ions of permethylated GSL): more product ions were observed from a double focusing mass spectrometer. Besides collision energy, the structure of the analyte had a significant effect on the formation of product ions. Despite the fact that masses of protonated molecular ions (MH + ) of permethylated GSL are significantly larger than their ceramide fragments, the low‐energy and high‐energy product ion spectra of MH + are, in general, similar. In a double focusing mass spectrometer of reversed geometry, more metastable ions were observed in the first field free region (FFR) than in the second FFR. The metastable ions observed in the second FFR were similar to those observed in low‐energy collision‐induced dissociation (CID). Although a double focusing mass spectrometer is superior to triple quadrupole instrument for detection of product ions, the poor resolution in either the selection of precursor ion or in the product ion spectra can be a serious problem in analysis of a mixture with similar masses.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here