Premium
New Pseudostationary Phases for Electrokinetic Chromatography: A High‐Molecular Surfactant and Proteins
Author(s) -
Terabe Shigeru,
Ozaki Hiroto,
Tanaka Yoshihide
Publication year - 1994
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199400036
Subject(s) - chemistry , micellar electrokinetic chromatography , chromatography , pulmonary surfactant , enantiomer , electrokinetic phenomena , micelle , methacrylate , acrylate , ionic strength , electrophoresis , copolymer , organic chemistry , polymer , aqueous solution , biochemistry
To extend the applicability of electrokinetic chromatography (EKC), two new types of pseudostationary phases have been introduced. A high‐molecular surfactant, butyl acrylate/butyl methacrylate/methacrylic acid copolymer (BBMA) is employed as a micellar forming surfactant for miccllar electrokinetic chromatography (MEKC). The critical micelle concentration of BBMA is essentially zero, which means the micellar concentration is constant irrespective of temperature and buffer. Some characteristic features of BBMA as the pseudostationary phase for MEKC is investigated in comparison with conventional ionic surfactants. Ovomucoid and avidin, which are proteins isolated from egg white, have been found to be useful chiral selectors in affinity EKC. A few examples of the separation of enantiomers with these proteins are shown.