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Side Reactions in Peptide Synthesis: Failure of Coupling in Solid‐Phase Automatic Synthesis of a Fragment (Sequence 65‐74) of the Acyl Carrier Protein
Author(s) -
Wu ChiYue,
Chen WenChao,
Yu HuiMing,
Chen ShuiTein,
Wang KungTsung
Publication year - 1992
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199200032
Subject(s) - chemistry , peptide , sequence (biology) , cleavage (geology) , peptide sequence , peptide synthesis , solid phase synthesis , amino acid , acyl carrier protein , combinatorial chemistry , peptide bond , peptide fragment , protein sequencing , stereochemistry , biochemistry , biosynthesis , enzyme , geotechnical engineering , fracture (geology) , engineering , gene
Two major side‐products and a desired peptide (each was one‐third) were found in analysis of a synthesized fragment (65‐74) of the acyl carrier protein by a solid‐phase automatic peptide synthesizer. The peptide mixtures were separated and collected by HPLC. Each isolated peptide was characterized via amino‐acid composition analysis, mass spectra and specific peptide‐bond cleavage to prove the sequence. Each by‐product had one amino‐acid missing ( 72 Ile and 73 Asn, respectively) from the sequence of ACP(65‐74).